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基于基因组组合和转录组比较的地下害虫Gryllotalpa Orientalis中化学传感基因的表征

Amna Chudhary¹, De-Long Guan², Yandi Xu¹

¹College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.

|January 14, 2025

概括

这项研究确定了东方 (Gryllotalpa orientalis) 中的化学感知基因,揭示了关键的气味受体和对其生存和害虫行为至关重要的结合蛋白. 这些发现有助于理解地下昆虫化学接收.

科学领域:

昆虫学昆虫学是一门学科.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

化学感官感知对于昆虫的生存至关重要,它影响了导航,交配和食等行为.
关键的基因家族包括臭味结合蛋白 (OBPs),嗅觉受体 (ORs),离子受体 (IRs),化学感知蛋白 (CSPs),味觉受体 (GRs) 和感知神经元膜蛋白 (SNMPs).
东方 (Gryllotalpa orientalis) 是一种入侵性害虫,由于它的地下生活方式,它会对作物造成重大破坏.

研究的目的:

为了描述Gryllotalpa orientalis的化学感知基因谱.
为未来关于这种物种化学接收的功能研究提供基础.
了解地下昆虫害虫中化学感知知觉的遗传基础.

主要方法:

在Gryllotalpa orientalis. 的新基因组组合.
转录组分析用于识别和量化基因表达.
生物信息分析以表征化学传感基因家族.

主要成果:

组装了G. orientalis的基因组草案,其中包含19,155个蛋白质编码基因,包括158个化学传感基因.
已确定的化学感知基因包括30个OR,64个IR,10个GR,28个OBP,25个CSP和1个SNMP.
表达分析显示了71个积极表达的化学感知基因,其中OR和OBP主要在头部和腿部,GR和IR在头部.

结论:

这项研究首次全面识别了G. orientalis基因组中的化学传感基因家族.
这些发现为未来对昆虫化学接收的功能研究奠定了基础.
这项研究强调了化学接收对于生活在地下环境中的昆虫的重要性.

关键词:

化学传感基因鉴定鉴定不同表达式的差异表达式基因组组装组的基因组组装组摩尔板球摩尔板球对转录组进行分析.

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