环CCT2/miR-146a-5p/IRAK1轴促进了头部和部状细胞癌的发展
Long He1,2, Lanruo Li3, Liting Zhao4,5
1Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, 030001, China. hl@sxent.org.
BMC cancer
|January 14, 2025
概括
循环RNACCT2 (CircCCT2) 通过调节miR-146a-5p/IRAK1通路,促进头皮状细胞癌 (HNSCC) 的发展. CircCCT2可以作为HNSCC的潜在生物标志物和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 头部和部状细胞癌 (HNSCC) 是一种具有不良结果的侵袭性癌症.
- 循环RNAs (circRNAs) 越来越多地被认为是各种癌症的关键调节者,包括HNSCC.
- 在HNSCC病变发生过程中,circRNAs的特定作用和机制需要进一步阐明.
研究的目的:
- 研究HNSCC中的circCCT2的表达和功能.
- 阐明circCCT2影响HNSCC进展的分子机制.
- 评估circCCT2作为HNSCC的潜在诊断生物标志物和治疗点.
主要方法:
- 定量实时PCR (qPCR) 用于测量HNSCC组织和细胞系中的circCCT2表达.
- 在体外测定 (CCK-8,殖民地形成,Transwell) 以评估扩散,迁移和入侵.
- 在体内异种移植瘤模型评估circCCT2.2的致癌作用.
- 机制研究包括双露西法酶记者测定,RNA免疫沉降 (RIP) 和西部涂抹,以确定目标相互作用.
主要成果:
- 与正常对照组相比,HNSCC组织和细胞中的CircCCT2表达显著上调.
- 升高的circCCT2水平与晚期瘤阶段,淋巴结转移和患者预后较差相关.
- 发现CircCCT2在体外和体内都能促进HNSCC细胞的增殖,迁移和入侵.
- 从机制上讲,circCCT2作为miR-146a-5p的竞争性内源RNA (ceRNA) 起作用,从而调节互白素-1受体关联激酶1 (IRAK1) 和促进表皮-介质细胞转换 (EMT).
结论:
- CircCCT2显著促进HNSCC的发展和进展.
- circCCT2的致癌功能通过miR-146a-5p/IRAK1轴进行介导,驱动EMT.
- CircCCT2代表了一个有前途的潜在生物标志物和HNSCC管理的治疗目标.
更多相关视频
相关概念视频
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
The Retinoblastoma Gene
4.0K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.0K
Induced Pluripotent Stem Cells
3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
3.9K
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K


