Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nuclear 2'-<i>O</i>-methylation regulates RNA splicing through its binding protein FUBP1.

Science advances·2025
Same author

Quantitative and Systematic NMR Measurements of Sequence-Dependent A-T Hoogsteen Dynamics in the DNA Double Helix.

Biochemistry·2025
Same author

A quantitative model predicts how m<sup>6</sup>A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions.

Nature communications·2021
查看所有相关文章

相关实验视频

Updated: Jun 2, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

6.6K

映射snoRNA目标转录组-宽与snoKARR-seq.

Bei Liu1

  • 1Department of Chemistry, The University of Chicago, Chicago, Illinois 60637, United States.

ACS chemical biology
|January 15, 2025
PubMed
概括

小核RNAs (snoRNAs) 指导RNA修饰,但其疾病作用尚不清楚. 一种新的方法,即snoRNA-enriched kethoxal-assisted RNA-RNA sequencing (snoKARR-seq),揭示了snoRNAs参与蛋白质转位和分泌.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生化学

背景情况:

  • 小核RNAs (snoRNAs) 是关键的非编码RNA,参与RNA化学修饰.
  • 斯诺RNA功能的全谱及其与人类疾病的联系尚未得到充分了解.
  • 探索snoRNAs的非正规角色对于理解它们的生物学意义至关重要.

研究的目的:

  • 开发一种高度敏感和特定的转录组全方位方法来识别snoRNA目标.
  • 为了研究超越RNA修饰的snoRNAs更广泛的细胞功能.
  • 探索snoRNAs在人类疾病中的潜在参与.

主要方法:

  • 开发和应用snoRNA丰富的托托哈素辅助RNA-RNA测序 (snoKARR-seq).
  • 全转录组分析以确定直接的snoRNA目标.
  • 使用生物化学和细胞分析验证新型snoRNA功能.

主要成果:

  • snoKARR-seq能够对snoRNA目标进行敏感和特定的发现.
  • 确定了snoRNAs在调节蛋白质转位中的意想不到的作用.
  • 在蛋白质分泌途径中证明了snoRNA的参与.

更多相关视频

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.1K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K

相关实验视频

Last Updated: Jun 2, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

6.6K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.1K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K
  • 扩大了已知的snoRNAs的功能表.

    • 结论:

    • snoKARR-seq是推动snoRNA研究的强大工具.
    • 斯诺RNA在蛋白质贩运中起着重要作用,影响细胞功能.
    • 这些发现扩大了对snoRNA生物学及其在疾病中的潜在影响的理解.