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ZjMAPKK4 与 ZjNAC78 相互作用调节朱树的冷耐受性反应

Qingfang Wang¹, Chaofeng Qi¹, Linxia Wang^1,2

¹College of Horticulture, Hebei Agricultural University, Baoding, Hebei, China.

Plant, cell & environment

|January 15, 2025

概括

朱果的寒冷耐受性是由ZjMAPKK4增强的,这种基因与ZjNAC78.8相互作用. 这种相互作用调节下游基因,提高植物承受寒冷压力的能力.

科学领域:

植物科学植物科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

朱 (Ziziphus jujuba Mill.) 是一种葡萄树,生长在南瓜树上. 是中国重要的水果作物,以干旱和盐水耐受性而闻名.
然而,由于对寒冷压力敏感,它的生长受到阻碍.
线素激活蛋白激酶 (MAPK) 级联的特定作用,特别是ZjMAPKK4,在朱的寒冷应激反应中并未得到充分理解.

研究的目的:

调查ZjMAPKK4在朱树对寒冷压力的反应中的功能.
阐明ZjMAPKK4调解寒冷耐受性的分子机制.
确定ZjMAPKK4参与冷应激信号的交互伙伴.

主要方法:

在冷处理下进行基因表达分析 (qRT-PCR).
在阿拉比多普西斯的异质表达式.
病毒诱导的基因沉默 (VIGS) 和在朱古力中过度表达.
转录组分析以确定差异表达基因 (DEG).
酵母两杂交,液体染色学-质谱学 (LC-MS) 和共免疫沉 (Co-IP) 试验.

主要成果:

在冷处理后,ZjMAPKK4的表达显著增加.
ZjMAPKK4积极调节朱的抗寒性,通过异质表达,VIGS和过度表达研究得到证实.
ZjMAPKK4与ZjNAC78.8进行相互作用.
沉默ZjNAC78导致寒冷敏感性和低调寒冷反应基因.
ZjMAPKK4和ZjNAC78调节了下游的ZjICE-ZjCBF基因.

结论:

ZjMAPKK4在朱古叶寒冷耐受性方面发挥着至关重要的积极作用.
ZjMAPKK4-ZjNAC78相互作用是朱树寒冷应激反应的关键调节途径.
这一途径涉及ZjICE-ZjCBF基因的下游调节,增强朱的抗寒能力.

关键词:

ZjMAPKK4 的时间 ZjNAC7878 的时间.的转化转化的转化寒冷的压力是冷的压力.朱朱贝贝 (朱朱贝贝) 是一个转录组 (transcriptome) 是一个转录组.

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