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晚期发育的试管受精牛胚胎囊中的亡增加降低了成功怀孕的可能性
Tae-Gyun Kim1, Yong-Ho Choe2, Sung-Ho Kim1
1College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Animal bioscience
|January 15, 2025
概括
胚胎质量影响牛的怀孕成功. 晚期发育的体外受精 (IVF) 囊在胚胎移植 (ET) 后显示出更高的亡和更低的怀孕率,这表明需要改进胚胎选择策略.
科学领域:
- 动物生物技术动物生物技术
- 生殖科学 生殖科学
- 胚胎学 胚胎学
背景情况:
- 胚胎质量对于胚胎移植 (ET) 后的牛成功怀孕至关重要.
- 在体外受精 (IVF) 胚胎囊在体外培养 (IVC) 过程中表现出不同的发育速度和怀孕建立潜力.
研究的目的:
- 为了比较早期,中期和晚期发育的牛体外受精胚胎囊 (7日,8日和9日) 的特征.
- 分析这些胚胎细胞的分子特征和怀孕能力之间的相关性.
主要方法:
- 胚芽细胞的形态评估 (细胞数量,直径)
- 对基因表达 (Oct4,Sox2,Bax,Bcl2,Tfam,Cox1) 和亡的分析.
- 胚胎囊特征与ET后怀孕率之间的相关性分析.
主要成果:
- 在第7天,第8天和第9天的胚胎囊之间没有观察到形态差异.
- 怀孕率显著下降,胚胎细胞发育延迟 (第7天:51.7%,第8天:36.7%,第9天:17.8%).
- 晚期发育的胚胎囊显示出亡的增加和关键发育基因的减少表达,与怀孕率有负相关性.
结论:
- 延迟的牛体外受精胚胎细胞发育与亡的增加和发育基因表达的减少有关.
- 这些发现支持改善牛ET的胚胎选择策略,以改善妊娠结果.
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