无线电频率诱导的基因表达和亡的时间依赖的变化在质母细胞瘤细胞系中
Mehmet Zahid Tuysuz1, Handan Kayhan2, Atiye Seda Yar Saglam3
1Department of Biophysics, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
Bioelectromagnetics
|January 15, 2025
概括
长时间的射频电磁场 (RF-EMF) 暴露,特别是48小时,显著影响质母细胞瘤细胞活力,并诱导细胞亡. 这项研究强调了无线设备长时间暴露在射频-电磁场的潜在风险.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 神经瘤学神经瘤学
背景情况:
- 无线技术增加了人类对射频电磁场 (RF-EMF) 的暴露.
- 大脑特别脆弱,因为它靠近手机和电信号传输.
- 质母细胞细胞 (U118-MG) 用于模拟脑瘤对RF-EMF暴露的反应.
研究的目的:
- 研究2.1 GHz射频辐射对质母细胞细胞活力,细胞亡和基因表达的影响.
- 为了确定不同暴露时间 (1,24小时和48小时) 对U118-MG细胞的影响.
- 分析特定的亡相关基因的作用和BAX/BCL-2比率.
主要方法:
- 为了在2.1 GHz进行细胞培养研究,开发了一种体外射频暴露系统.
- 特定吸收率 (SAR) 通过数值剂量计 (1.12 ± 0.18 W/kg) 确定.
- 细胞活力,细胞亡标志物 (酶水平) 和基因表达 (BAX/BCL-2比率) 在1,24小时和48小时后进行评估.
主要成果:
- 对2.1 GHz射频场的48小时暴露显著影响了U118-MG细胞活力和基因表达.
- 在射频暴露24小时和48小时后,观察到CASP3,CASP8和CASP9的mRNA水平增加.
- 细胞亡和BAX/BCL-2比率的显著升高仅在暴露48小时后才明显.
结论:
- 长时间的射频电磁场暴露 (48小时) 可以诱导质母细胞瘤细胞的亡.
- BAX/BCL-2比率和细胞翻倍时间可能会影响对射频辐射的亡反应.
- 这些发现表明,射频场对脑瘤细胞的潜在生物效应,需要进一步调查.
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