尤根醇在癌前乳腺病变中抑制NEAT1作为ceRNA
Yusheng Liu1,2, Guijuan Zhang2, Yi Ma3
1Department of Traditional Chinese Medicine, Yangjiang People's Hospital, Yangjiang, Guangdong, 529525, China.
Heliyon
|January 15, 2025
概括
叶绿醇 (EU) 是一种来自丁香的化合物,在治疗癌前乳腺病变 (PBL) 中表现有前途. 它通过降低NEAT1,VEGF-A和MMP-9的调节,同时提高miR-383-5p和miR-9-5p的调节,从而提供了一个潜在的治疗策略.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 长非编码RNAs (lncRNAs) 作为竞争的内源RNAs (ceRNAs) 起作用,在转录后网络中调节微RNAs (miRNAs).
- 来自丁香的尤根醇 (EU) 具有显著的抗瘤特性.
- 癌前乳腺病变 (PBL) 是乳腺癌发展的重要阶段.
研究的目的:
- 调查尤金醇 (EU) 对癌前乳腺病变 (PBL) 的影响.
- 阐明lncRNA-miRNA-mRNA ceRNA网络在欧盟抗瘤活性中的作用.
- 为了分析包括NEAT1,miR-383-5p,miR-9-5p,MMP-9和VEGF-A在内的关键分子的表达,以响应欧盟治疗.
主要方法:
- 来自NCBI数据库的基因表达特征的生物信息分析 (n=880).
- 一个lncRNA-miRNA-mRNA ceRNA网络的构建.
- 使用MCF-10AT细胞的体外研究和使用PBL大鼠模型的体内研究.
- 定量逆转录聚合酶连锁反应 (RT-qPCR),西式涂抹和免疫组织化学染色以评估基因和蛋白质表达.
主要成果:
- 构建了一个ceRNA网络,涉及3个lncRNA,3个miRNA和38个mRNA,确定了1162个mRNA,81个miRNA和26个lncRNA作为乳腺癌和癌前乳腺癌的趋势基因.
- 发现NEAT1,miR-383-5p,miR-9-5p,VEGF-A和MMP-9在乳腺瘤细胞中的下调.
- 欧盟治疗抑制了MCF-10AT细胞生长,降低了NEAT1,VEGF-A和MMP-9水平,并在体外和体内增加了miR-383-5p和miR-9-5p的表达.
结论:
- 欧原醇 (EU) 通过调节涉及miR-383-5p和miR-9-5p的NEAT1介导的ceRNA网络,有效抑制癌前乳腺病变 (PBL).
- 欧盟通过这种途径减少了VEGF-A和MMP-9的表达.
- 欧盟证明了作为PBL治疗剂的潜力.
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