使用低成本3D打印进行可定制的大规模HPLC分量采集
William J Crandall1, Marco Caputo2, Lewis Marquez1,3
1Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA.
HardwareX
|January 15, 2025
概括
研究人员开发了一种低成本,可定制的分量收集器,用于高性能液态染色学 (HPLC) 系统. 这种3D打印设备提高了可扩展分数收集的可复制性和可访问性.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 高性能液态色谱 (HPLC) 是分子分离的关键技术.
- 自动化分量采集系统提高了可重复性,但通常是昂贵和不灵活的.
- 现有的商业分数收集器对研究人员的可访问性和定制性构成重大障碍.
研究的目的:
- 为HPLC系统提供一个经济实惠且可定制的微分采集器的高效构造.
- 克服商业分量采集器的局限性,包括成本,定制和体积容量.
- 提供一个平台,提高可扩展和代分数收集方法的可重复性.
主要方法:
- 使用业余级3D打印机 (Creality Ender 3 Pro) 和挤出用于施工.
- 开发了一个图形用户界面 (GUI),用于用户友好地编程没有编码的收集方法.
- 设计该系统与各种HPLC系统兼容,并支持大量的收集量.
主要成果:
- 成功构建了一个低成本 (<280美元) 可定制的HPLC微分采集器.
- 与商业替代品相比,支持显著更大的收集量 (高达470毫升).
- 图形界面使操作简单,不需要先前的编码经验来编程方法.
结论:
- 开发的3D打印分数采集器为HPLC应用提供了高度可定制和具有成本效益的解决方案.
- 这个平台消除了成本障碍,并增加了需要可扩展的分数收集的研究人员的可访问性.
- 该系统增强了准备规模分数采集方法的可重复性.
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