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Updated: Jun 2, 2025

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

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作为向细胞输送蛋白质的通用方式

Kathleen H Ngo1,2, Max E Distler1,2, Michael Evangelopoulos3,2

  • 1Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.

Journal of the American Chemical Society
|January 15, 2025
PubMed
概括

改善蛋白质输入细胞需要创建混合的DNA树突蛋白结构. 与传统的球状核酸 (proSNA) 纳米结构相比,这些新型结构显著增强了细胞吸收.

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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