在RAW 264.7细胞中对除草剂的体外免疫毒性评估
Larissa Vivan Cestonaro1,2, Aline Mocellin Conte1,2, Fernanda Capitanio Goldoni3
1Laboratório de Toxicologia, Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Journal of toxicology and environmental health. Part A
|January 15, 2025
概括
这项研究表明,常见的除草剂,如克洛马,甘酸盐和硫酸盐可以伤害免疫细胞. 这些农业化学物质诱导氧化应激并抑制免疫反应,影响免疫系统的整体健康.
科学领域:
- 农业科学 农业科学
- 毒理学 毒理学 毒理学
- 免疫学 免疫学 免疫学
背景情况:
- 除草剂对于农业的杂草管理至关重要.
- 新兴的除草剂耐药性需要新的化学解决方案.
- 除草剂对非标细胞的潜在免疫毒性作用需要调查.
研究的目的:
- 评估克洛马,草甘和硫的细胞免疫毒性.
- 调查涉及除草剂暴露的毒理途径.
- 为了评估这些除草剂对RAW 264.7巨细胞的影响.
主要方法:
- 在96小时内用除草剂化RAW 264.7小鼠巨细胞系.
- 使用EC50值评估细胞毒性作用.
- 测量反应性氧物种 (ROS) 和反应性物种 (RNS) 产量.
- 分析线粒体去极化和瘤亡因子-α (TNF-α) 水平.
主要成果:
- 克洛马,草甘和硫酸盐表现出具有不同EC50值的细胞毒性作用.
- 克洛马和硫松显著增加了ROS和RNS的产生.
- 在特定度下,草甘和硫氨酸在RAW 264.7细胞中诱导免疫抑制.
- 所有测试的除草剂都引起了线粒体脱极化,并降低了TNF-α水平.
结论:
- 克洛马和硫拉首次在RAW 264.7细胞上显示出免疫毒性.
- 除草剂可以通过氧化应激和减少细胞因子的产生对免疫细胞功能产生不利影响.
- 这些发现强调了需要进一步研究广泛使用的除草剂的免疫毒理学特征.
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