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卡诺辛EXTRA®会导致线粒体生物能反应在MCF-7和MRC-5细胞系中的变化

Aleksandra Popović¹, Jovana Drljača Lero², Dejan Miljković³

¹Faculty of Medicine Novi Sad, Department of Physiology, University of Novi Sad, Novi Sad, Serbia.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

|January 15, 2025

概括

来自Karnozin EXTRA®的L-carnosine会影响肺纤维细胞和乳腺癌细胞中的细胞活力和线粒体呼吸. 这项研究揭示了瘤代谢和能量通路的潜在影响,需要进一步研究.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

L-卡诺因其在调节瘤生长和细胞代谢方面的潜在作用而闻名.
了解L-carnosine对线粒体功能和氧化应激的影响对于癌症研究至关重要.

研究的目的:

研究卡诺辛EXTRA® (L-卡诺辛) 对人类肺纤维细胞 (MRC-5) 和乳腺癌细胞 (MCF-7) 中的线粒体呼吸链复合体的影响.
分析L-carnosine对细胞增殖,氧化应激标志物和细胞能量通路的影响.

主要方法:

在24小时内对MRC-5和MCF-7细胞系用不同度的L-carnosine (2,5,10mM) 进行治疗.
评估细胞活力,形态和增殖指数.
通过光和免疫阳性对氧化应激标志物 (SOD2,iNOS,CYP2E1) 的分析.
使用克拉克型电极测量线粒体呼吸 (基底,最大,与ATP相关) 和呼吸链复合体 (I,II,IV) 的活性.

主要成果:

L-卡诺辛在两种细胞系中都表现出剂量依赖的细胞活性的降低和细胞形态的改变.
在MRC-5和MCF-7细胞之间观察到对SOD2表达的不同影响;iNOS免疫阳性因度而异.
在所有实验组中都注意到CYP2E1 (氧化应激标志物) 的表达增加.
基本细胞呼吸,电子运输链容量,ATP相关呼吸和复合体I,II和IV活动的显著减少.

结论:

卡诺辛EXTRA® (L-卡诺辛) 在正常肺纤维细胞和乳腺癌细胞中显著影响线粒体呼吸和关键酶活动.
这些发现表明,L-卡诺作为细胞能量代谢的调节剂的潜力,影响细胞活力和氧化应激.
这些结果为进一步的临床前和临床研究L-carnosine的治疗潜力提供了基础.

关键词:

乳腺癌乳腺癌乳腺癌这就是SOD2的原因.卡诺西尼 (Carnosine) 是一种类型的肉素.我们的 iNOS iNOS 它们的扩散和扩散.呼吸链中的呼吸系统.

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