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与血管内皮生长因子A和与calcitonin基因相关的基因相关的tenomodulin的时间表达和特定定位在小鼠发育的隔膜中
Ryutaro Nakagawa1, Iwao Sato2, Yoko Ueda3
1Department of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Anatomical science international
|January 15, 2025
概括
膜发育过程中,模素 (TNMD) 的表达集中在肌结处. 相比之下,血管内皮生长因子A (VEGF-A) 和素基因相关 (CGRP) 分布广泛.
科学领域:
- 发展生物学 发展生物学
- 连接组织生物学 连接组织生物学
- 分子生物学分子生物学
背景情况:
- 模素 (TNMD) 是一种来自软骨的生长调节剂,在肌等低血管连接组织中发现.
- 血管内皮生长因子A (VEGF-A) 和素基因相关 (CGRP) 分别参与血管生成和神经生成.
- 在隔膜发育过程中,TNMD,VEGF-A和CGRP的精确分布尚不清楚.
研究的目的:
- 研究TNMD,VEGF-A和CGRP的mRNA和蛋白质表达模式.
- 绘制它们在发展中的隔膜中央肌 (CTD) 和小鼠周围组织中的分布图.
主要方法:
- 使用in situ杂交和免疫组织化学来可视化蛋白质和mRNA的表达.
- 采用定量实时聚合酶连锁反应用于基因表达分析.
- 从各种胚胎 (E14.5,E17.5) 和产后 (P1,P5) 阶段的小鼠身上检查的组织.
主要成果:
- 在胚胎第17.5天 (E17.5) 和出生后第1天 (P1) 中,TNMD在CTD的肌结处显示出偏好的局部化.
- 从胚胎第14.5天 (E14.5) 到产后第5天 (P5) 的VEGF-A和CGRP在整个隔膜中呈现分散的分布.
结论:
- TNMD的表达特异地定位在腹膜中正在发展的肌肉结处.
- 独特的表达模式表明TNMD,VEGF-A和CGRP在CTD发展中的独特作用.
- 这些发现为TNMD在隔膜中央肌的形成中的作用提供了基础的见解.
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