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Updated: Jul 17, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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建立基于NFSWI-DDA模式的新型大规模向代谢学方法,使用HRMS和TQ-MS

Rongrong Li1, Xinyi Jiao1, Xiaolin Wu2

  • 1State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin, 301617, PR China; Haihe Laboratory of Modern Chinese Medicine, Tianjin, 301617, PR China.

Talanta
|January 15, 2025
PubMed
概括

通过一种新的非固定细分窗口间隔数据依赖获取 (NFSWI-DDA) 方法,提高了非目标代谢物识别. 这种方法增强了MS2的覆盖范围,以准确识别代谢物和在生物样本中进行有针对性的分析.

关键词:
这就是LC-MS.大规模的大规模.这是一个MRMM.在 NFSWI-DDA 中.有针对性的代谢组学.

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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...