VvATG18a参与了葡萄对由BR信号通路诱导的灰色菌的抵抗
Run-Yu Zhou1, Jia-Yan Qu1, Hui-Ping Niu1
1College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China.
International journal of biological macromolecules
|January 15, 2025
概括
铜类固醇 (BRs) 通过调节自,增强葡萄的抗病能力. 这项研究确定了VvBZR1与VvATG18a促进体结合,增强了对灰色菌的宿主防御.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 植物免疫力 植物免疫力
背景情况:
- 自对于植物对病原体和激素信号的防御至关重要.
- 铜类固醇 (BRs) 是一种调节葡萄抗病能力的植物激素.
- 葡萄对灰色菌的防御中的BRs和自之间的分子联系尚未完全理解.
研究的目的:
- 阐明葡萄在对灰色菌的防御中brassinosteroid-autophagy网络的分子机制.
- 为了研究类固醇在激活葡萄宿主防御反应中的作用.
主要方法:
- 在葡萄上对24-epibrassinolide (eBR) 的外源性应用.
- 转录组分析以确定差异表达基因 (DEG).
- 用DNA亲和力净化测序 (Dap-seq),酵母单杂交 (Y1H) 和双光酶测定 (LUC) 来验证基因相互作用.
主要成果:
- eBR的应用增强了葡萄的抗病能力,并增加了内源性BR水平.
- 转录组分析显示,在EBR处理的葡萄中,BR信号和自途径的丰富.
- 证实VvBZR1可以结合VvATG18a促进体,诱导其表达.
- 过度表达VvATG18a和VvBZR1显著改善了对灰色菌的抵抗力.
结论:
- 鉴定了一种新型的防御机制,涉及葡萄中的BR调节的自细胞对灰色菌的防御.
- VvATG18a在增强葡萄天生的免疫力和抗病能力方面发挥着关键作用.
- 这项研究加深了对自在植物免疫反应中的作用的理解.
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