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一个染色体层次的基因组组装和注释的脂肪绿 (Hexagrammos otakii)

Li Li¹, Fawen Hu², Dong Liu¹

¹Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao), Qingdao, 266104, China.

Scientific data

|January 15, 2025

概括

研究人员已经测序了脂肪绿 (Hexagrammos otakii) 的第一个染色体级基因组,这是一个关键的水产养殖鱼. 这种基因组资源将有助于改善疾病耐药性和可持续农业增长等特征.

科学领域:

海洋基因组学海洋基因组学
水产养殖遗传学水产养殖遗传学
进行比较基因组学.

背景情况:

脂肪绿 (Hexagrammos otakii) 在中国北方水产养殖中具有经济重要性.
挑战包括特征退化,早期的性成熟和疾病易感性.
需要基因组资源来实现可持续的种植和选择性育种.

研究的目的:

为了生成第一个染色体水平的基因组组合为Hexagrammos otakii.
为H. otakii水产养殖改善提供基础基因组资源.
为了支持繁殖计划,生殖细胞保护和遗传研究.

主要方法:

这是一种PacBio长读测序技术.
用于脚手架的 Hi-C 技术.
用于基因组完整性评估的BUSCO分析.
蛋白质编码基因预测和功能注释.

主要成果:

组装了一个682.43 Mb的基因组大小,具有高contig (2.39 Mb) 和脚手架 (27.83 Mb) N50值.
基因组完整性通过BUSCO.99%被评估为96.99%.
预测了22334个编码蛋白质的基因,其中96.80%的功能注释.

结论:

这项研究介绍了首个高质量的染色体层次的脂肪绿化基因组组.
这种基因组资源对于理解H. otakii遗传学至关重要.
它将促进加强水产养殖,包括抗病和增长优化等战略的制定.

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