相关实验视频

Updated: Jun 2, 2025

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

开伊果的基因组序列草案,这是一个未充分利用的非洲树作物作物

Robert Kariba¹, Bernice Waweru^2,3, Isaac Njaci^2,4

¹World Agroforestry, P.O Box 30677, Nairobi, 00100, Kenya. R.Kariba@cifor-icraf.org.

Scientific data

|January 15, 2025

概括

凯果 (Dovyalis afra) 的基因组被测序,为改善粮食和营养安全提供了一个资源. 这棵非洲本土的果树显示出适应气候的农业和改善生计的潜力.

科学领域:

基因组学就是基因组学.
农业食品系统农业食品系统
营养科学营养科学

背景情况:

全球粮食和营养安全受到人口增长和气候变化的威胁.
像凯果这样的土著果树,尽管具有弹性和营养密度,但仍未得到充分利用.
整合这些被忽视的作物对于可持续的农业食品系统至关重要.

研究的目的:

介绍凯果 (Dovyalis afra) 的基因组序列草案.
为释放本土非洲果树的潜力提供基因组资源.
支持粮食安全,营养和气候适应性农业的努力.

主要方法:

使用了一种基于长时间阅读的基因组组装方法.
基因组组装覆盖了1190个连接中的440 Mbp.
通过基因组注释识别了编码蛋白质的基因.

主要成果:

成功生成了凯果 (Dovyalis afra) 的基因组序列草案.
该组件的N50值为13.3Mbp,L50值为11.
总共有27,449个蛋白质编码基因被确定.

结论:

凯果基因组序列是未来研究的宝贵资源.
这些基因组数据可以帮助提高粮食安全和营养应用.
该研究支持将被忽视的本土果树纳入农业食品系统.

更多相关视频

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Genome Size and the Evolution of New Genes

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.

Genomic DNA in Eukaryotes

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...