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Updated: Jun 2, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

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转录调节元件的大规模并行表征

Vikram Agarwal1,2, Fumitaka Inoue3,4,5, Max Schubach6

  • 1Department of Genome Sciences, University of Washington, Seattle, WA, USA. vikram.agarwal@sanofi.com.

Nature
|January 15, 2025
PubMed
概括

研究人员使用基于lentivirus的大规模并行报告测试 (lentiMPRAs) 绘制了数百万个cis-regulatory元素 (cCREs). 这项研究揭示了控制cCRE活动和细胞类型特异性的序列特征,进步了我们对基因调控的理解.

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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