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再组合蛋白质被乳球球菌 (Lactococcus cremoris) 纳入细胞外囊泡
Tina Vida Plavec1,2, Kristina Žagar Soderžnik3, Giulia Della Pelle3
1Department of Biotechnology, Jožef Stefan Institute, Ljubljana, Slovenia.
Scientific reports
|January 15, 2025
概括
乳酸细菌Lactococcus cremoris NZ9000可以产生携带重组蛋白质的细胞外囊泡 (EV). 这些工程细菌EV显示了由于细菌的可扩展治疗性蛋白质输送的潜力.
科学领域:
- 微生物学和生物技术
- 细胞外囊泡 (EVs) 研究研究
- 细菌纳米粒子生产方法
背景情况:
- 细胞外囊泡 (EVs) 对于细胞间通信至关重要,并有可能用于诊断和药物输送.
- 与哺乳动物的EV相比,对细菌EV的研究,特别是来自像Lactococcus cremoris这样的克拉姆阳性细菌的研究,是较少的.
- 了解安全细菌菌株的EV生产是生物技术应用的关键.
研究的目的:
- 为了研究乳球球菌 (Lactococcus cremoris) 的细胞外囊泡 (EV) 生产NZ9000.
- 分析重组蛋白表达对EV形成和货物的影响.
- 评估工程L. cremoris EVs在治疗性蛋白质输送方面的潜力.
主要方法:
- 用L. cremoris NZ9000的基因工程来表达四种不同的重组蛋白质.
- 使用标准超离心法隔离细胞外囊泡 (EVs).
- 通过传输电子显微镜,流动细胞计和质谱仪对电动汽车的表征.
主要成果:
- 在孤立样本中确认了50-200nm的囊泡结构.
- 质谱检测证实了EV中存在重组蛋白 (13.17-100%) 和本地L. cremoris蛋白的存在.
- 蛋白质加载到EV似乎不受管制,但可扩展的重组蛋白质含有EV的生产是可行的.
结论:
- 乳球球菌克雷莫里斯NZ9000可以被设计成产生载有重组蛋白质的细胞外囊泡 (EVs).
- 这些细菌EV提供了一个可扩展的平台来提供治疗性蛋白质.
- L. cremoris NZ9000的GRAS地位为开发使用其EVs的新生物技术和治疗应用提供了优势.
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