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肺内皮细胞衰老损害了屏障功能,促进了中性粒细胞的粘附和迁移

Maliheh Najari Beidokhti¹, Nuria Villalba¹, Yonggang Ma¹

¹Department of Molecular Pharmacology and Physiology, University of South Florida, Morsani College of Medicine, 12901 Bruce B. Downs Blvd., Tampa, FL, USA.

|January 15, 2025

概括

肺内皮细胞中的细胞衰老会削弱细胞结点和屏障功能. 这促进了炎症和中性粒细胞的迁移,有助于与年龄相关的肺功能障碍.

科学领域:

老年学是指老年学的学科.
细胞生物学细胞生物学
肺部病理学肺部病理学

背景情况:

细胞衰老,标志着不可逆转的细胞循环停止,有助于与衰老相关的炎症和器官功能障碍.
衰老在不同细胞类型和器官中表现出各种形态和功能影响.

研究的目的:

为了研究老年肺和肺内皮细胞 (ECs) 中的细胞衰老标志物.
确定肺部EC衰老对屏障完整性和炎症细胞相互作用的功能影响.

主要方法:

在老年人和老鼠肺部和培养老鼠肺部EC中检查了衰老标志物 (p21, γH2AX, SA-β-Gal, Ki-67, Lamin B1).
在培养的EC中使用SAHA或DOXO诱导衰老.
通过溶液透性评估了EC屏障功能,并评估了中性粒细胞化学反应和迁移.

主要成果:

衰老的肺和衰老的EC显示p21,γH2AX,SA-β-Gal的增加,Ki-67,Lamin B1的减少.
衰老与肺炎,中性粒细胞透和改变的连接蛋白 (VE-cadherin,ZO-1) 相相关.
衰老的ECs显示屏障功能受损,并促进了中性粒细胞的迁移.

结论:

肺部EC衰老通过削弱细胞-细胞结合来损害内皮屏障的完整性.
衰老的肺部ECs通过促进中性粒细胞的粘附和迁移,促进炎症.
肺部EC衰老可能会导致与年龄相关的肺炎和病理.

关键词:

衰老的衰老衰老的衰老内皮细胞是内皮细胞.炎症炎症是一种炎症.中性粒细胞中性粒细胞.透性透性的衰老是一种衰老.

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