在小麦 (Triticum aestivum L.) 叶病原发生过程中,阐明竞争的内源性调节性RNA网络的circRNA-miRNA-mRNA
Umang Kumar Dhandhanya1, Uzma Afreen1, Shailendra Kumar Jha2
1Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.
Functional & integrative genomics
|January 15, 2025
概括
小麦叶的抗性涉及复杂的非编码RNA相互作用. 这项研究揭示了循环RNA和微RNA如何调节防御基因,增强作物对病原体的抵抗力.
科学领域:
- 植物科学 植物科学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 非编码RNAs (ncRNAs) 越来越多地被认为是它们在基因调节中的作用.
- 了解ncRNA功能对于开发具有增强抗病能力的作物至关重要.
- 小麦叶生对全球粮食安全构成重大威胁.
研究的目的:
- 研究小麦中微RNA (miRNA) 和圆形RNA (circRNA) 在叶子生感染期间的功能相互作用.
- 构建和验证参与小麦防御反应的竞争性内源RNA (ceRNA) 网络.
- 阐明叶子抗基因Lr28在这个调节网络中的作用.
主要方法:
- 从易受和耐受性小麦近同源线 (NILs) 获得miRNAs和circRNAs的差异表达分析.
- 在线预测circRNA-miRNA和miRNA-mRNA相互作用.
- 使用RT-qPCR构建和验证一个ceRNA调控网络.
- 对circRNA,miRNA和mRNA表达特征的相关性分析.
主要成果:
- 确定了一个ceRNA网络,涉及四个差异表达的circRNA (DEC) 和五个差异表达的miRNA (DEM).
- 证明了这个网络在对五种防御反应基因 (mRNAs) 对叶子生的升级中发挥的关键作用.
- 确认了circRNAs和mRNAs的协同表达,与miRNAs有敌对关系.
- 突出了Lr28基因在增强circRNA介导目标基因上调中的作用.
结论:
- 环RNA-miRNA-mRNA调节网络在小麦抵御叶子生方面发挥着至关重要的作用.
- Lr28显著影响circRNAs在促进疾病耐药性目标基因表达的效率.
- 这项研究为识别生物标志物提供了洞察力,通过多omics方法改善小麦的弹性.
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