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Updated: Jun 2, 2025

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使用富里埃图形显微镜进行无边界工件定量相位成像

Yao Jin, Yefeng Shu, Yuzhen Zhang

|January 16, 2025

概括

里埃图形显微镜 (FPM) 边界文物使用新的边界延伸重建框架 (BE-FPM) 减少. 这种方法提高了数字病理学和细胞生物学应用的定量阶段成像 (QPI) 质量.

科学领域:

显微镜和成像技术技术.
生物物理学的生物物理.
数字病理学数字病理学

背景情况:

里埃图形显微镜 (FPM) 提供高分辨率,广场振幅和相位成像.
在FPM重建中的边界文物降低了图像质量和相位检索精度.
这些文物源于非周期数据和光衍射上的快速里埃变换 (FFT).

研究的目的:

引入一个新的框架,边界扩展FPM (BE-FPM),用于无文物定量相位成像 (QPI).
解决和从根本上解决FPM中的边界文物问题.
为改进的FPM重建提供计算效率高的解决方案.

主要方法:

为FPM开发了一个边界延伸重建框架 (BE-FPM).
在代过程中初始化了零填充的重建,并采用了自我推算.
启用了超出图像边界的样本信息的部分恢复.

主要成果:

使用BE-FPM实现了无边界工件的QPI.
与现有方法相比,演示了最小的计算开销.
成功地将BE-FPM应用于微镜阵列和活细胞成像.

结论:

BE-FPM有效地消除了FPM中的边界工件.
该方法确保了在边界周围的样本一致性,以便准确地表征相位.
BE-FPM是一个多功能和有效的解决方案,用于各种FPM应用.

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