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相关概念视频

X-ray Crystallography02:18

X-ray Crystallography

23.8K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
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X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Jun 2, 2025

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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使用自参照模块进行光学衍射断层扫描.

Zhengyuan Tang1, Julianna Winnik2, Bryan M Hennelly1,3

  • 1Department of Electronic Engineering, Maynooth University, Maynooth, Co. Kildare, Ireland.

Biomedical optics express
|January 16, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种新且具有成本效益的光学衍射断层扫描 (ODT) 系统,用于无标签的3D折射率成像. 这种模块化系统可以与现有显微镜集成,并提供用于生物样本分析的双成像模式.

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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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相关实验视频

Last Updated: Jun 2, 2025

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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科学领域:

  • 生物医学光学 生物医学光学
  • 细胞成像 细胞成像
  • 全息影像的使用方法.

背景情况:

  • 无标签成像对于研究活生物样本至关重要.
  • 目前的光学衍射断层扫描 (ODT) 系统可能是复杂而昂贵的.
  • 需要具有适应性和成本效益的ODT解决方案.

研究的目的:

  • 提出一种新的,模块化的,具有成本效益的光学衍射断层扫描 (ODT) 系统.
  • 为了实现无标签的3D折射率 (RI) 生物样本的成像.
  • 将ODT与现有的生命科学显微镜集成在一起.

主要方法:

  • 一个模块化的两部分系统:带有加尔沃扫描仪的照明模块和自参照全息捕捉模块.
  • 自动对齐协议用于与标准生命科学显微镜无集成.
  • 采用自我引用的离轴全息以获取数据.

主要成果:

  • 展示了一个紧的,具有成本效益的ODT系统,具有简单的对齐.
  • 实现了无标签,3D RI成像能力.
  • 能够在ODT和实时合成光圈数字全息显微镜 (SA-DHM) 模式之间切换.
  • 使用癌细胞系进行实验验证.

结论:

  • 开发的模块化ODT系统为3D RI成像提供了具有成本效益和多功能性的解决方案.
  • 它与标准显微镜的兼容性和双模特功能增强了它在生物研究中的实用性.
  • 这种方法促进了先进的无标签细胞分析.