为高脂质和高粉生产设计新型极端藻Chlamydomonas pacifica作为开发商业相关菌株的途径

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Abhishek Gupta¹, João Vitor Dutra Molino¹, Kathryn M J Wnuk-Fink²

¹Department of Molecular Biology, School of Biological Sciences, University of California San Diego, La Jolla, San Diego, California 92093, United States.

ACS ES&T engineering

|January 16, 2025

概括

极端友好型的微藻Chlamydomonas pacifica被基因改造,以提高脂质和粉的生产. 这项研究表明它们在可扩展的生物燃料和生物材料生产方面的潜力,支持可持续的生物经济.

科学领域:

生物技术是生物技术.
藻类生物技术藻类生物技术
可持续的生物经济

背景情况:

微藻是生物产品的高效生产者,但面临着可扩展性的挑战.
极度友好型微藻提供了对恶劣条件的弹性,使其成为工业应用的有希望的产品.
目前的微藻种植受生物质生产成本和池撞击脆弱性限制.

研究的目的:

开发一条基因工程和从极端爱好微藻中生产生物柴油和聚氨前体的试点规模生产管道.
为了提高Chlamydomonas pacifica的弹性和生产力,用于工业生物产品的生产.
为了证明工程极端友微藻的商业可行性,以实现可持续的生物经济.

主要方法:

突变,繁殖和选择被用来进化Chlamydomonas pacifica,使其具有高光耐受性.
基因工程被用来增加C. pacifica.中的脂质和粉积累.
在赛道池中进行了试点规模的种植,并将生物质转化为生物柴油和热塑性聚氨.

主要成果:

改造后的C. pacifica的脂质产量增加了28%,粉积累增加了27%,但没有影响生长速度.
进化后的菌株对极端条件有很高的耐受性 (pH>11.5,2%NaCl,42°C,光>2000μE/m2/s).
取得了成功的试点规模的培养和生物质转化为生物柴油和热塑性聚氨.

结论:

极端友好型微藻,特别是C. pacifica,可以被改造为有价值的生产菌株,用于商品生物制品.
这项研究介绍了从发现到试点规模生产的完整管道,为生物经济验证极端友的藻类.
工程化C. pacifica为生物燃料和生物材料生产提供了可持续的解决方案,解决了环境挑战和市场需求.

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