在纸厂使用安普利康序列测序在活性污泥中对 prokaryote 和 eukaryote 的表征
Ignacio López Bravo1, María Fernanda Dominguez2,3, Ana Claudia Mionetto1
1Montes del Plata, Colonia, Uruguay.
Heliyon
|January 16, 2025
概括
转基因编码揭示了纸厂激活污泥中的微生物群体,确定了关键的真核生物和原核生物群体. 这种DNA测序方法为监测和改进纸和造纸行业的废水处理提供了强大的工具.
科学领域:
- 环境微生物学 环境微生物学
- 分子生态学分子生态学
- 生物技术是生物技术.
背景情况:
- 纸和造纸行业的废水处理依赖于活性污泥 (AS) 系统.
- 目前的监测方法与化学参数的相关性有限.
- 微生物群落的组成显著影响AS反应器的效率.
研究的目的:
- 在纸厂AS中使用metabarcoding全面描述 prokaryotic 和 eukaryotic 微生物群落.
- 评估元编码作为废水处理中常规微生物社区监测的工具.
- 为了改善环境管理,建立AS中微生物群落的基准.
主要方法:
- 使用MiniON和Illumina测序进行16S (核细胞) 和18S (真核细胞) 子测序.
- 在一家纸厂的扩展通风激活污泥中分析了微生物群落.
- 将元编码结果与传统的基于显微镜的评估进行了比较.
主要成果:
- 由原生体 (Ciliophora),Rotifers (Animalia) 和真菌 (Cryptomycota) 主导的真核社区.
- Prokaryotic 社区揭示了诸如 Terrimonas,Defluviicoccus 和 Methylocaldum 这样的主导属.
- 超级编码提供了比显微镜更深入的见解,显示了一致的分类学趋势,并突出了特定的主导群体.
结论:
- 元编码是一种可行且强大的工具,用于在纸厂AS.的微生物社区综合分析.
- 鉴定 prokaryotic 和 eukaryotic 社区的特征对于有效的反应堆运行和环境管理至关重要.
- 这项研究为微生物群落建立了基线,为将微生物数据与过程模式联系起来铺平了道路.
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