显明的Spirocerca lupi通过使用植物遗传学和植物地理学分析在美洲传播
Paula Alfaro-Segura1, Joby Robleto-Quesada1, Víctor M Montenegro-Hidalgo2
1Laboratory of Helminthology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica.
Frontiers in parasitology
|January 16, 2025
概括
一种危险的狗寄生虫Spirocerca lupi在美洲进行了基因分析. 美国的Spirocerca lupi菌株属于1型基因组,这表明亚洲起源可能是由迁徙宿主传播的.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子遗传学分子遗传学
- 狗狗健康 狗狗健康
背景情况:
- 螺旋虫 (Spirocerca lupi) 是一种全球分布的寄生性线虫,影响着类动物.
- 它引起食道螺旋,可能导致严重的疾病,如癌瘤和大动脉动脉瘤.
- 之前对Spirocerca lupi的基因型分析排除了来自美洲的标本.
研究的目的:
- 来自北美和中美洲的Spirocerca lupi标本的分子特征.
- 确定美洲斯皮罗塞卡卢皮的遗传关系和进化历史.
- 为了调查Spirocerca lupi基因型的起源和分散模式.
主要方法:
- 使用18SrDNA和细胞染色体c氧化酶子单元1 (cox1) 基因片段对Spirocerca lupi的分子表征.
- 遗传学分析包括贝叶斯推断 (BI) 和坦普尔顿-克兰达尔-辛格 (TCS) 哈普罗型网络.
- 对cox1基因的族群地理分析,以推断进化历史.
主要成果:
- 贝叶斯推断 cox1 遗传树将美国的 Spirocerca lupi 与以色列标本分组在一起,将它们分类为基因型 1.
- TCS单双型网络显示,美国标本与以色列Spirocerca lupi.密切结合.
- 18S rDNA基因片段将哥斯达黎加虫与非洲,亚洲和欧洲的虫以及其他螺旋虫种类区分开来.
结论:
- 来自美洲的Spirocerca lupi属于基因型1,与以色列分离物有着密切的遗传相似性.
- 遗传学分析表明,螺旋虫起源于欧洲,分化为螺旋虫,这些螺旋虫连续传播到非洲,亚洲和美洲.
- 斯皮罗塞卡卢皮在美洲的存在很可能是由于受感染的宿主从亚洲分散.
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