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Chromosome Structure02:40

Chromosome Structure

22.5K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.5K
Karyotyping01:17

Karyotyping

57.1K
Overview
57.1K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
DNA Microarrays02:34

DNA Microarrays

17.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K
Euchromatin01:01

Euchromatin

6.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.8K
Heterochromatin02:38

Heterochromatin

10.4K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
10.4K

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相关实验视频

Updated: Jun 2, 2025

Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

2.3K

绘制3D基因组架构的地图.

Ghazaleh Tavallaee1, Elias Orouji1

  • 1Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.

Computational and structural biotechnology journal
|January 16, 2025
PubMed
概括

本综述探讨了绘制基因组3D架构的方法. 与传统方法相比,基因组架构映射 (GAM) 和SPRITE等无结合技术可以更好地了解复杂的染色质结构.

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因组的空间组织对于基因表达,细胞分化和基因组稳定性至关重要.
  • 了解三维 (3D) 基因组架构对于破译基本生物过程至关重要.

研究的目的:

  • 提供对绘制3D基因组架构的方法的深入审查.
  • 为了比较传统的基于结合的技术与先进的无结合的方法.
  • 讨论3D基因组数据与其他基因组层和单细胞技术的整合.

主要方法:

  • 基于结合的基因组构造捕获技术的检查.
  • 分析无绑定方法,包括基因组架构映射 (GAM) 和通过标签扩展 (SPRITE) 分分池识别相互作用.
  • 讨论用于分析染色体相互作用的多模式方法和单细胞技术 (sci-HiC, scSPRITE).

主要成果:

  • 像GAM和SPRITE这样的无结合方法克服了传统技术的局限性,例如限制酶偏差和结合效率低下.
  • 这些先进的方法可以捕捉复杂的,更高阶的染色体相互作用.
  • 多模式和单细胞方法揭示了在发育和疾病期间染色体结构的异质性.
关键词:
3D基因组架构的3D基因组架构染色是一种染色素.捕获染色体形状的捕获方式表观基因组学是指表观基因组学.这就是Hi-C.单细胞基因组学 单细胞基因组学

更多相关视频

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

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相关实验视频

Last Updated: Jun 2, 2025

Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

2.3K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

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结论:

  • 无结合技术在解决复杂的3D基因组结构方面取得了重大进展.
  • 整合多样化的基因组数据和采用单细胞分辨率是理解生物背景下基因组组织的关键.
  • 这些方法的进一步开发将提高我们对健康和疾病中的基因组调节的理解.