快速和自动解释基于CRISPR-Cas13的横向流量测试结果,使用机器学习
Mengyuan Xue1, Diego H Gonzalez1, Emmanuel Osikpa2
1Department of Bioengineering, Rice University Houston TX 77030 USA lillehoj@rice.edu.
Sensors & diagnostics
|January 16, 2025
概括
使用神经网络自动解释CRISPR-Cas侧流检测 (LFA),提高了诊断准确度. 机器学习模型可以准确地从智能手机图像中读取LFA结果,从而提高临床检测的可靠性.
科学领域:
- 生物技术是生物技术.
- 人工智能的人工智能
- 分子诊断学 分子诊断学
背景情况:
- 基于CRISPR-Cas的侧流检测 (LFA) 提供了敏感的核酸检测.
- 对LFA的视觉解释可能会导致人为错误和不准确的结果.
- 需要自动解释来提高CRISPR-Cas LFA的可靠性.
研究的目的:
- 开发和验证神经网络模型,用于基于CRISPR-Cas的LFA结果的自动解释.
- 在各种条件下使用智能手机拍摄的图像来评估这些模型的准确性.
- 通过使用CRISPR-Cas技术实现准确可靠的临床诊断.
主要方法:
- 开发了两个神经网络模型:一个完全卷积网络和一个移动优化的网络.
- 应用模型来解释基于CRISPR-Cas13的LFA用于SARS-CoV-2 N基因检测.
- 经过训练和验证的模型在3146个LFA设备的智能手机图像上,具有不同的图像质量和背景.
主要成果:
- 神经网络模型在分类LFA结果时实现了96.5%的准确性.
- 自动解释是快速执行的,每次测试在0.2秒内.
- 模型在各种图像定向,背景,照明和质量方面表现出强度.
结论:
- 机器学习可以准确地从智能手机图像中解释基于CRISPR-Cas的LFA结果.
- 自动解释提高了CRISPR-Cas诊断工具的可靠性和实际应用.
- 这项技术支持准确的自我和家庭测试,提高了诊断的可访问性.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...