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在酵母溶酶样真空体中的V-ATPase分解是复制性衰老中溶酶功能障碍的表型驱动因素

Fiza Hashmi¹, Patricia M Kane¹

¹Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA.

|January 16, 2025

概括

随着年龄的增长,由于V-ATPase的分解,溶解体酸性降低. 卡路里限制和维护RAVE复杂水平可以防止这种情况,从而保持细胞功能并延长寿命.

科学领域:

细胞生物学细胞生物学
衰老研究研究衰老研究
分子生物学分子生物学

背景情况:

溶酶体酸化对细胞功能至关重要,并且随着年龄的增长而下降.
V-ATPases是有机体pH的关键调节者,它们的活性通过可逆分解来调节.
衰老会影响包括酵母在内的各种生物,影响细胞过程.

研究的目的:

用Saccharomyces cerevisiae研究V-ATPase分解在细胞衰老中的作用.
为了确定调节V-ATPase在老化过程中的组装和拆卸的因素.
探索热量限制对与年龄相关的V-ATPase功能障碍的影响.

主要方法:

利用Saccharomyces cerevisiae作为一个复制性衰老模型.
使用显微镜和生物化学分析分析了V-ATPase分解和真空 pH.
研究了真空体和内体酸化调节剂 (RAVE) 复合体和Oxr1在V-ATPase调节中的作用.

主要成果:

在老化的酵母细胞中,V-ATPases分解成V1和V0亚复合体,导致真空的化.
卡路里限制阻止了V-ATPase的分解,并维持了老细胞中真空 pH 稳态.
RAVE复合物促进组装,而Oxr1促进拆卸;减少RAVE亚单元Rav2在老细胞中的水平与拆卸相关.

结论:

减少V-ATPase组合有助于与年龄相关的溶酶体酸化损失.
通过像RAVE和Oxr1这样的因素调节V-ATPase组装/拆卸,会影响细胞寿命.
像热量限制这样的干预措施可以保持V-ATPase功能并减轻衰老的影响.

关键词:

这种植物是Saccharomyces cerevisiae.老化的老化衰老的老化热量限制热量限制溶解体是溶解体的组成部分.质子的质子.

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