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Updated: Jun 2, 2025

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
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通过ExoScreen技术进行细胞外囊分泌相关基因查的协议

Tomofumi Yamamoto1, Fumihiko Urabe2, Yusuke Yoshioka3

  • 1Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan; Laboratory of Integrative Oncology, National Cancer Center Research Institute, Tokyo, Japan; Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Kawasaki, Kanagawa, Japan.

STAR protocols
|January 16, 2025
PubMed
概括

这项研究提出了一种结合微RNA库和ExoScreen技术的新方法,用于选涉及细胞外囊泡 (EV) 分泌的基因,并识别miRNA标,帮助癌症研究.

关键词:
细胞生物学 细胞生物学基于细胞的测试分子生物学分子生物学

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Overview of Exosomes01:36

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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