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在斑马鱼胚胎生成期间的翻译控制中,5' UTRs的监管格局

Madalena M Reimão-Pinto¹, Sebastian M Castillo-Hair², Georg Seelig³

¹Biozentrum, University of Basel, 4056 Basel, Switzerland.

Developmental cell

|January 16, 2025

概括

这项研究揭示了信使RNA5'未翻译区域 (5' UTRs) 如何在斑马鱼发育过程中控制基因翻译. 我们确定了调节核糖体招募的特定序列动机和特征,为翻译启动动态提供了定量模型.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

传递 RNA (mRNA) 的 5' 未翻译区域 (5' UTR) 对于调节胚胎发育期间的基因翻译至关重要.
然而,它们的特定的体内调节功能和机制在很大程度上仍未被描述.

研究的目的:

在早期斑马鱼胚胎发生过程中全面地绘制5' UTRs的监管格局.
在体内识别影响翻译启动和核糖体招募的序列动机和特征.
开发一个定量模型,预测5' UTR介导的翻译调节.

主要方法:

在 18,154 个 5' UTR 序列上使用了大规模并行记者分析 (MPRA).
集成的MPRA与多胞体造型,以评估核糖体占用率.
开发了一个深度学习模型,Danio Optimus 5-Prime (DaniO5P),用于定量预测.

主要成果:

证明了单独的5' UTRs可以赋予对翻译启动的时间控制.
在5' UTR中识别了86个序列动图,表现出明显的核糖体招募能力.
丹尼O5P模型揭示了5' UTR长度,翻译启动地点上下文,上游AUG和序列动机共同影响核糖体招募.

结论:

5' UTR 长度和图案组合对开发过程中翻译启动的动态有着显著的贡献.
本文介绍了第一个基于5' UTR的翻译调节在发展背景下的定量模型.
提供了识别调节过程中介于这些调节过程的分子因素的基础.

关键词:

5′ UTR 5′ UTR 的时间表.深度学习模型深度学习模型大规模并行记者测试.控制翻译启动的控制斑马鱼的胚胎发生

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