相关实验视频

Updated: Jul 19, 2026

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Published on: December 22, 2010

表面涂层纳米架构学,以优化细胞兼容性和抗菌活性:酸定位作为最外层的影响

Guilherme L B Neto¹, Tiago R B Quinalia¹, Débora A de Almeida¹

¹Department of Chemistry, State University of Maringá, Maringá, PR, Brazil; Laboratory of Materials, Macromolecules, and Composites, Federal University of Technology - Paraná, Apucarana, PR, Brazil.

International journal of biological macromolecules

|January 16, 2025

概括

使用 hialuronic acid (HA) 和 poly (diallyldimethylammonium chloride) (PDDA) 的多重电解质多层 (PEM) 增强了聚乙烯 (PS) 的湿透性,并表现出抗菌特性. 这两种PEM都显示出细胞相容性,这表明生物医学应用的潜力,如假肢涂层.

科学领域:

材料科学材料科学材料科学
生物材料工程生物材料工程
表面化学表面化学

背景情况:

多电解质多层 (PEM) 为各种应用提供可调整的表面性能.
氨酸 (HA) 和聚 (二氧化二甲基化物) (PDDA) 是生物相容的多电解质,具有表面修饰的潜力.
氧化聚钢 (PS_ox) 是表面功能化的常见基质.

研究的目的:

在氧化聚氨酸 (PS_ox) 上制造和描述由HA和PDDA组成的PEM.
评估PEMs对PS_ox的湿透性和表面粗性的影响.
评估制造PEMs的抗菌活性和细胞兼容性,以潜在的生物医学应用.

主要方法:

层次沉积 (LbL) 被用来在PS_ox上创建HA/PDDA PEM.
用X射线光电子光谱 (XPS) 和原子力显微镜 (AFM) 来进行表面表征.
接触角测量评估了可湿性.
使用JIS Z 2801-2010标准对*P. aeruginosa*和*S. aureus*进行了抗菌活性评估.
使用红细胞和人类脂肪衍生干细胞 (ADSC) 测试了细胞兼容性.

主要成果:

PEM沉积被XPS证实,AFM显示表面粗度增加.
PEMs显著提高了PS的湿透性,接触角度从73° (PS) 减少到24° (PDDA终止) 和36° (HA终止).
终止HA的PEM显示了对*P. aeruginosa*和*S. aureus*的抗菌活性.
与HA终止的PEM相比,PDDA终止的PEM对*S. aureus*表现出更高的抗菌抑制 (48%),而HA终止的PEM则高 (32%).
这两种PEM类型都表现出与红细胞和ADSCs的良好细胞相容性.

结论:

HA/PDDA PEM 有效地改变了PS_ox的表面特性,提高了可湿性.
制造的PEM具有显著的抗菌活性,特别是PDDA终结的变种.
这些PEM的细胞相容性表明它们适用于生物医学应用,包括假肢涂层.

关键词:

血液血液血液血液聚甲基化物 (Poly ((diallyldimethylammonium 化物)) 是一种聚甲基化物.多重电解质多层聚合物.

更多相关视频

Graphene Coatings for Biomedical Implants

Graphene Coatings for Biomedical Implants

Published on: March 1, 2013

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating

Published on: July 8, 2016

相关概念视频

Glycocalyx and its Functions

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...

Outer Layers of the Cell Envelope

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...