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通过本地分离物增强细菌纤维素生产:从突变发生和进化技术的见解

Mohammad Rezaei¹, Mehrdad Azin¹, Davood Zare¹

¹Iranian Research Organization for Science and Technology (IROST), Sh. Ehsani Rad St., Enqelab St., Ahmadabad Mostoufi Rd., Azadegan Highway, P. O. Box 33535-111, Tehran 3313193685, Iran.

International journal of biological macromolecules

|January 16, 2025

概括

本土细菌纤维素分离物显著提高了生产效率. 优化菌株的产量是细菌纤维素的六倍以上,这对于包装和伤口包裹等环保应用至关重要.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
微生物学微生物学

背景情况:

细菌纤维素 (BC) 具有理想的性能,如机械强度和高吸水能力,使其适用于包装,伤口带和药物输送.
工业规模的BC生产面临着低效率和高成本的挑战,需要高产品种和优化条件.

研究的目的:

识别和增强本土细菌菌株,以生产优质细菌纤维素.
提高细菌纤维素合成的效率和产量,用于工业应用.

主要方法:

选土著细菌分离物,特别是来自的细菌分离物.
紫外线突变和适应性实验室进化 (ALE) 的应用,以改善菌株.
使用扫描电子显微镜 (SEM),X射线衍射 (XRD) 和富里埃变换红外光谱 (FTIR) 进行细菌纤维素的表征.

主要成果:

一个本地菌株PP177480 (与Komagataeibacter sucrofermentans密切相关) 的生产率达到9.3g/L,比标准菌株的1.31g/L有显著提高.
紫外线突变和ALE导致生产效率提高了六倍以上.
在SEM中,所选菌株产生的纤维素中发现了更大的纳米孔尺寸;XRD证实了纤维素I结构,具有不同的晶体体尺寸和结晶率百分比.

结论:

本土细菌分离物为高产细菌纤维素生产提供了一个有希望的来源.
通过突变发生和ALE优化菌株可以克服工业生产的局限性.
增强的细菌纤维素具有适合先进应用的结构特征.

关键词:

适应性实验室进化的演变.细菌纤维素的细菌纤维素.隔离隔离隔离隔离 K. xylinusinus 是一个类型.突变突变是一种突变.

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