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Updated: Jun 2, 2025

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

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在多发性硬化症患者中通过聚合酶链反应和流细胞计检测STAT4

Dina A Mohareb¹, Ahmad K Mostafa¹, Mai M Nosair¹

¹Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

The Egyptian journal of immunology

|January 17, 2025

概括

这就是STAT4基因.

科学领域:

神经免疫学神经免疫学
多发性硬化症的遗传学

背景情况:

多发性硬化症 (MS) 涉及免疫系统对中枢神经系统髓的攻击.
STAT4基因变异与增加的MS风险和改变的免疫反应有关.

研究的目的:

调查STAT4基因多态和蛋白质水平与活性MS疾病之间的关联.
评估STAT4作为MS活动的潜在生物标志物.

主要方法:

使用限制片段长度多态聚合酶链反应 (RFLP-PCR) 进行基因型鉴定.
通过流细胞计量量化STAT4蛋白水平的量化.
80名多发性硬化患者和70名健康对照人群之间的比较.

主要成果:

在MS患者中,STAT4基因型GC和CC,以及C等位基因,比对照患者更为普遍.
STAT4 GC基因型和C等位基因与MS疾病活性显著相关.
与不活跃患者和对照患者相比,在活跃的MS患者中观察到较高的STAT4蛋白水平.

结论:

STAT4基因多态和蛋白质表达与MS疾病活性有关.
流细胞计检测白细胞中的STAT4蛋白质可以作为监测MS进展的生物标志物.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...