相关实验视频

Updated: Jul 13, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

来自与Ilex pubescens相关的行为菌体的自然产品和生物活性. 这是Arn & Arn

Xinyue Zhang¹, Zhaoyang Tao¹, Jianshu Chen¹

¹The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.

Natural product research

|January 17, 2025

概括

来自Ilex pubescens的内性细菌Streptomyces nanningensis产生了五种化合物. 热尼斯和酸显示出对大肠杆菌和白杆菌的抗菌活性,具有抗氧化特性.

科学领域:

自然产品化学自然产品化学
微生物学微生物学
药理学药理学是一个学科.

背景情况:

内生性动因菌种,如Streptomyces nanningensis,是生物活性天然产品的来源.
伊莱克斯青春期是一种因其潜在的药用特性而闻名的植物.

研究的目的:

为了隔离和识别来自Ilex pubescens中发现的内性Streptomyces nanningensis的化合物.
评估分离化合物的抗微生物和抗氧化活性.

主要方法:

从Ilex pubescens的叶子中分离了内性动因菌体.
使用乙烯酸乙烯提取和净化化合物.
使用核磁共振 (NMR) 和质谱法 (MS) 阐明结构.
确定针对大肠杆菌和白杆菌的最小抑制度 (MIC).
评估DPPH的自由基清除活动.

主要成果:

确定了五种化合物:热尼斯 (1),埃斯库莱丁 (2),乌尔索酸 (3),原酸 (4) 和醇酸 (5).
热尼斯 (1) 和原酸 (4) 对大肠杆菌表现出抗菌活性 (MIC = 0.7815 mg/mL).
原酸 (4) 显示出对白虫的抑制作用 (MIC = 0.7815 mg/mL).
热尼斯 (1) 和酸 (4) 显示了DPPH自由基清除活性,其IC50值分别为125微克/毫升和63微克/毫升.

结论:

从Ilex pubescens中分离出来的Streptomyces nanningensis产生生物活性化合物.
热尼斯和酸具有显著的抗微生物和抗氧化特性,需要进一步研究治疗应用.

关键词:

伊莱克斯 pubescensens 的时间.这些是actinomycetes.生物活动是生物活动.二次代谢产物二次代谢产物

更多相关视频

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method

Published on: June 14, 2020

Isolation of Soil Microorganisms Using iChip Technology

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

相关概念视频

Bacterial Phylum Actinobacteria

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...

Microbe-Plant Interactions

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...