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对代谢学和转录学的综合分析显示,在Gloriosa品种中,有各种各样的tepal色素

Yue Sun¹, Pinli Hu¹, Yanan Jiang¹

¹Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, China.

BMC plant biology

|January 17, 2025

概括

这项研究揭示了胡卜素和酸盐如何创造多样化的Gloriosa花的颜色. 确定了关键的基因和转录因子,解释了黄色,色,红色和紫色的变异.

科学领域:

植物科学植物科学
分子生物学分子生物学
代谢学代谢学代谢学

背景情况:

由于其广泛的花色彩,Gloriosa L. 具有显著的装饰价值.
了解这些颜色变化的遗传和代谢基础对于育种和园艺至关重要.

研究的目的:

为了研究Gloriosa的tepal颜色的表现形式表达.
为了分析胡卜素和氨酸的组成.
为了确定参与色素生物合成的差异表达基因.

主要方法:

颜色测量和超高性能液态染色学-并联质谱学 (UPLC-MS/MS) 用于色素分析.
转录组测序和权重基因联合表达网络分析 (WGCNA) 用于基因表达概况.
定量实时PCR (qRT-PCR) 用于基因表达的验证.

主要成果:

胡卜素 (例如β-胡卜素,黄蛋白) 是黄色/色的关键,而安东素 (氨酸,氨酸,氨酸) 驱动色,红色和紫色.
分别确定了57个和92个候选基因在胡卜素和氨酸通路中分别表达 (DEGs).
发现了转录因子 (TF) 和色素生物合成基因之间显著的共同表达网络,其中一个特定的MYB基因与酸盐水平相关.

结论:

由特定的基因和TFs调节的胡卜素和氨酸积累,决定了Gloriosa的多样化的花颜色.
这项研究阐明了Gloriosa多变的眼色彩背后的分子机制.
研究结果为具有理想装饰性特征的Gloriosa品种的有针对性的育种提供了洞察力.

关键词:

格洛里奥萨 (Gloriosa) 是一个光荣的城市.安托氨酸是一种安托氨酸.胡卜素是一种胡卜素.花的颜色花的颜色代谢组中的代谢.转录组转录组就是一个转录组.

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