含有药物的水凝微针,可持续通过皮肤输送宏分子蛋白质
Rubhan Chandran1,2, Eusni Rahayu Mohd Tohit1, Johnson Stanslas3
1Haematology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Current drug delivery
|January 17, 2025
概括
修改pH的聚乙烯乙 (甲基乙烯和酸) 水凝微针 (HMN) 能够直接加载牛血清白蛋白 (BSA) 等蛋白质. 这种方法增强了胀,并保留了蛋白质结构,以持续通过皮肤递送.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 聚合物化学 聚合物化学
背景情况:
- 聚甲乙烯合马氏酸 (PMVE/MA) 水凝微针 (HMN) 由于生物相容性和胀,具有通过皮肤传递宏分子药物的潜力.
- 药物被困在HMN矩阵和从外部储库扩展的扩散途径限制了输送效率,特别是在高分子量药物中.
研究的目的:
- 通过结合pH修改步骤,开发PMVE/MA HMN的直接药物加载方法.
- 为了研究pH值修改对HMN物理化学性质和蛋白质稳定性的影响.
- 为了评估蛋白质载荷的HMN通过皮肤传递的疗效,ex vivo.
主要方法:
- 通过pH调整步骤修改了PMVE/MA HMN.
- 分析了改性HMN的物理化学特性,包括胀行为和形态学.
- 牛血清白蛋白 (BSA) 被加载到pH修饰的HMN中,并评估其结构完整性.
- 评估了BSA载荷的HMN的ex vivo通过皮肤递送.
主要成果:
- pH值的改变显著增加了HMN的胀 (p < 0.001) 并产生了多孔的水凝结构.
- ATR-FTIR分析证实了碳酸峰的变化,表明pH值的修改是成功的.
- 在加载到pH修饰的HMN后,BSA的二次结构仍然保持不变.
- 带有BSA的HMN表现出持续的体外释放,在24小时内达到64.70%的累积释放.
结论:
- 经pH修改的PMVE/MA HMN系统可促进直接的蛋白质加载.
- 这种方法增强了水凝的特性,改善了药物封装和释放.
- 开发的HMN系统显示了可持续的通过皮肤递送蛋白质的前景.
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