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Funneliformis mosseae BR221 的效应蛋白:通过基于参考的转录组分析,体外验证和蛋白质-蛋白质对接研究来解开植物-真菌相互作用

Pratima Vasistha¹, Pushplata Prasad Singh², Divya Srivastava¹

¹Deakin Nanobiotechnology Centre, TERI, Sustainable Agriculture Division, TERI Gram, The Energy and Resources Institute, Gwal Pahari, Gurgaon Faridabad Road, Gurgaon, Haryana, 122001, India.

|January 17, 2025

概括

树菌 (AM) 菌分泌效应蛋白,调节宿主相互作用. 在Funneliformis mosseae BR221中识别这些作用因子,有助于我们更好地理解植物和真菌的共生,从而实现可持续农业.

科学领域:

菌类学菌类学是指菌类学.
植物与真菌的相互作用
分子生物学分子生物学

背景情况:

树状菌根菌 (AM) 是各种生态系统中至关重要的共生生物.
抗菌真菌分泌效应物来操纵宿主细胞,抑制防御,并促进营养的获取.
了解这些分子相互作用是优化AM真菌应用的关键.

研究的目的:

为了识别和描述来自AM真菌Funneliformis mosseae BR221.1.的效应蛋白.
阐明AM真菌与宿主相互作用背后的分子机制.
为增强农业中AM真菌应用提供基础.

主要方法:

Funneliformis mosseae BR221.的基于参考的转录组测序
在使用病原体与宿主相互作用数据库的效应蛋白的in silico预测.
蛋白质与蛋白质对接分析,以预测与植物蛋白质的相互作用.

主要成果:

在F. mosseae BR221中发现了163种效应蛋白,其中很大一部分是细胞外的.
预计四个候选效应因子对于建立AM真菌与宿主相互作用至关重要.
预测的效应因子与参与免疫,水合,营养吸收和应激反应的植物蛋白相互作用.

结论:

这项研究提供了对由效应因子介导的真菌与宿主分子相互作用的基础见解.
这些发现为选择特定的AM真菌菌株铺平了道路,为生物肥料量身定制的效应器.
这项研究通过增强作物弹性和营养吸收来支持可持续农业实践的发展.

关键词:

形形形形形形形形形形形形树冠状菌菌菌 (AM菌) 的真菌.影响者是影响者.微生物与宿主相互作用分泌蛋白质是分泌蛋白质的组成部分.这是一种共生关系.

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