骨头位的体积生物打印
Jessie Duquesne1, Laurens Parmentier1, Edward Vermeersch1
1Polymer Chemistry and Biomaterials (PBM) Group, Centre of Macromolecular Chemistry (CMaC), Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Krijgslaan 281, Building S4, 9000 Ghent, Belgium.
这项研究引入了一种新的骨质生化生物墨水 (GelNBNBSH) 用于体积生物打印,与现有方法相比,它显示出优异的细胞成熟和构造特性. 这一进步促进了骨组织工程支架的更快,更有效的生物制造.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 卷度生物打印可以快速制造大型生活结构.
- 需要多种不同的骨质生化生物墨水来促进结构内的细胞成熟.
- 现有的生物墨水通常需要高的光启动剂度和光剂量,这可能会影响细胞活力.
研究的目的:
- 开发和评估一种用于体积生物打印的新型骨质原生生物墨水.
- 为了比较一步增长生物墨水 (GelNBNBSH) 与链增长生物墨水 (GelMA) 的性能.
- 评估开发的生物墨的骨质生成潜力和材料特性.
主要方法:
- 使用norbornene-norbornene功能化凝和硫化凝 (GelNBNBSH) 进行阶段生长的生物ink.
- 将GelNBNBSH与凝甲基烯基 (GelMA) 生物墨水在不同的光启动器度和光照下进行比较.
- 进行了物理化学表征,包括降解研究和机械测试.
- 进行了21天的体外骨质细胞研究,使用牙纸衍生干细胞.
主要成果:
- 凝NBNBSH需要比GelMA低3倍的光启动器度和>50%的光暴露,从而提高分辨率.
- 选择的Li-TPO-L度 (1 mg ml-1) 实现了骨质基质弹性和>95%的细胞活力.
- 由于固有的纤维状结构,体积生物打印导致了较慢的降解.
- 与GelMA构造相比,GelNBNBSH构造显示了2倍高的交叉链接分量转换和3倍大的刚性.
- 在GelNBNBSH中封装的细胞表现出增强的早期和晚期骨质生成标志物 (ALP活性,产量).
- 秘密组分析显示,在GelNBNBSH构造中,一个更成熟的骨质生成,免疫调节和血管生成的表型.
结论:
- 凝NBNBSH生物墨水是骨质构造体积生物打印的一个有前途的材料.
- 逐步增长的聚合提供了优势,减少了光启动器和光剂量要求.
- 开发的生物墨水支持强大的细胞活力,并促进增强的骨质分化和成熟.
- 这项技术对骨组织工程应用具有重大潜力.
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