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相关实验视频

Updated: Jun 1, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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一个单一的微流体装置用于多omics分析样品准备.

Ranjith Kumar Ravi Kumar1, Iman Haddad1, Massamba Mbacké Ndiaye1

  • 1Spectrométrie de Masse Biologique et Protéomique SMBP, ESPCI Paris, LPC CNRS UMR 8249, PSL University, 10 Rue Vauquelin, F-75005 Paris, France. yann.verdier@espci.fr.

Lab on a chip
|January 17, 2025
PubMed
概括
此摘要是机器生成的。

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这项研究引入了使用ChipFilter设备进行微生物样本同时进行蛋白质和基因组分析的新工作流. 这种方法通过从单个样本中对蛋白质和核酸进行并行分析,从而减少偏差,从而提高了对微生物群的理解.

科学领域:

  • 微生物学 微生物学
  • 生物技术是生物技术.
  • 分析化学 分析化学

背景情况:

  • 综合微生物组分析需要整合多omics数据,如基因组学和蛋白质组学.
  • 准确的样本处理和分析方法对于可靠的微生物组数据至关重要.
  • 此前,ChipFilter设备已被验证用于微生物蛋白质组分析.

研究的目的:

  • 从相同的微生物样本中开发和验证用于同时进行蛋白质组和基因组分析的工作流.
  • 评估ChipFilter设备在准备样本进行并行奥米克分析时的有效性.
  • 评估工作流对样本偏差的影响,特别是对于少量样本.

主要方法:

  • 使用ChipFilter设备同时进行微生物培养物的溶解和蛋白质分解 (大肠杆菌,B. subtilis,S. cerevisiae).
  • 进行了基于液体染色学-并联质谱 (LC-MS/MS) 的蛋白质组分析的回收.
  • 使用聚合酶链反应 (PCR) 恢复和放大DNA以进行随后的基因组分析.
  • 将工作流应用于复杂的微生物混合物,并将DNA测序结果与商用工具包进行比较.

主要成果:

  • 在ChipFilter处理后使用LC-MS/MS成功识别了微生物蛋白质.

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相关实验视频

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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  • 从用于蛋白质组分析的相同样本中成功实现了DNA放大和测序.
  • 在复杂的微生物混合物中识别了超过5000种蛋白质.
  • DNA测序性能与使用商业套件提取的DNA相当,即使之前进行了蛋白解.

    • 结论:

    • 芯片过器适用于准备样本进行并行蛋白质和基因组分析.
    • 这种集成的工作流特别有利于分析少量微生物样本.
    • 该方法显著减少了多omics微生物组研究中的采样偏差.