转录激活和联合激活剂结合由酵母Ino2和人类原蛋白蛋白c-Myc结合
Eva-Carina Wendegatz1, Julia Lettow1, Wiktoria Wierzbicka1
1Center for Functional Genomics of Microbes, Institut Für Genetik Und Funktionelle Genomforschung, Universität Greifswald, Felix-Hausdorff-Straße 8, 17487, Greifswald, Germany.
Current genetics
|January 17, 2025
概括
人类c-Myc/Max蛋白和酵母Ino2/Ino4蛋白在进化上是相关的. 虽然Myc/Max可以激活酵母基因,但它们不能完全取代Ino2/Ino4,这表明转录重新连接.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 癌症生物学 癌症生物学
背景情况:
- 酵母Ino2/Ino4中的基本螺旋环-螺旋环 (bHLH) 域调节脂生物合成基因.
- 参与细胞增殖的人类c-Myc/Max蛋白与Ino2/Ino4.4具有结构上的相似性.
研究的目的:
- 为了功能性地比较酵母Ino2/Ino4和人类c-Myc/Max蛋白质作为潜在的正义基因.
- 研究这些转录因子异构体之间的进化关系和功能分歧.
主要方法:
- 在Saccharomyces cerevisiae中稳定合成c-Myc和Max.
- 评估通过c-Myc/Max.对Ino2/Ino4目标基因的激活.
- 双重突变的功能补充测定.
- 在c-Myc.中映射转录激活域 (TADs).
- 用c-Myc TADs进行同活性剂结合试验.
主要成果:
- c-Myc和Max在酵母中稳定合成,并激活了Ino2/Ino4.4的目标基因.
- c-Myc/Max无法功能地补充一个双重突变.
- 在c-Myc.中确定了两个转录激活域 (TAD1: aa 1-41,TAD2: aa 91-140).
- 酵母联合激活剂 (TFIID子单元,染色体重塑剂) 与c-Myc TADs结合,类似于Ino2 TADs.
结论:
- 酵母Ino2/Ino4和人类c-Myc/Max异构体在进化上是相关的.
- 尽管与协同激活剂保持相互作用,但c-Myc/Max和Ino2/Ino4经历了转录性重新连接,导致不同的基因调节.
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