基于生物信息学的免疫相关基因研究和急性心肌梗塞的临床验证
1Department of Emergency, The Wenzhou Third Clinical Institute Affiliated To Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou Maternal and Child Health Care Hospital, Wenzhou, 325000, Zhejiang, China.
Biochemical genetics
|January 17, 2025
概括
在急性心肌梗塞 (AMI) 患者中,三个基因CLEC4D,CLEC4E和LY96显著过度表达. 这些基因可以作为预测AMI发生和进展的关键生物标志物.
科学领域:
- 心血管生物学 心血管生物学
- 基因组学就是基因组学.
- 生物标志物发现发现
背景情况:
- 急性心肌梗塞 (AMI) 是一种严重的心血管疾病,由冠状动脉狭窄和硬化引起.
- 确定可靠的生物标志物对于AMI的早期诊断和管理至关重要.
研究的目的:
- 确定能够预测或影响急性心肌梗塞 (AMI) 进展的新型基因.
- 探索已识别的基因作为AMI的诊断生物标志物的潜力.
主要方法:
- 使用的基因表达综合 (GEO) 数据集 (GSE109048,GSE123342) 用于分析.
- 应用权重基因相关性网络分析 (WGCNA) 和蛋白质与蛋白质相互作用 (PPI) 网络分析.
- 使用机器学习算法和qRT-PCR进行基因验证.
主要成果:
- 通过WGCNA和PPI网络分析确定了154个关键基因和18个核心基因.
- 三个基因 (CLEC4D,CLEC4E,LY96) 被确定为潜在的AMI预测因子.
- 在AMI患者中,CLEC4D,CLEC4E和LY96显示出显著的过度表达,与免疫细胞透相关.
结论:
- 与健康对照组和冠状动脉心脏病 (CAD) 患者相比,AMI患者的CLEC4D,CLEC4E和LY96显著上调.
- 这些基因显示出作为急性心肌梗塞预测生物标志物的潜力.
- 这些发现表明CLEC4D,CLEC4E和LY96在AMI病原和免疫反应中的作用.
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