相关实验视频

Updated: Jun 15, 2026

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Published on: October 16, 2014

在引起周围假肢关节感染的物种中微生物生长和生物膜形成的差异:一项动物研究

Mehmet Batu Ertan¹, Mehmet Yağız Ayduğan², Ebru Evren³

¹Department of Orthopedics and Traumatology, Atılım Unıversity School of Medicine, Ankara, Turkey. mbatuertan@gmail.com.

International microbiology : the official journal of the Spanish Society for Microbiology

|January 17, 2025

概括

黄金葡萄球菌 (Staphylococcus aureus) 在周围假肢关节感染 (PJIs) 中显示了植入物上的最高感染负荷. 这表明,在最初的3-4周内,除菌,抗生素和植入物保留 (DAIR) 可能对S. aureus PJIs的有效性较低.

科学领域:

生物医学工程生物医学工程
传染性疾病传染性疾病
整形外科手术整形外科手术

背景情况:

周围假肢关节感染 (PJIs) 是关节整形术后的严重并发症.
常见的外科干预包括除bridement,抗生素和植入物保留 (DAIR) 和修复关节整形.
治疗选择取决于生物膜成熟,需要了解微生物生长动态.

研究的目的:

评估常见PJI病原体的生物膜形成和微生物生长.
为了在体内模型中比较植入物表面上的微生物发育.

主要方法:

一个体内研究,使用40只斯普拉格-道利大鼠,分为五个组 (金黄色,S. epidermidis,P. aeruginosa,C. albicans,控制).
合金盘被植入皮下并用微生物接种.
盘子和软组织的培养每两周收集一次,持续6周,以评估微生物负载和生物膜结构.

主要成果:

在整个研究中,黄金葡萄球菌在植入物盘上表现出最高的感染负载.
从第4周开始,在软组织中观察到显著的黄金色杆菌感染负载.
显微镜分析显示,在测试的微生物群体中,生物膜结构没有明显的差异.

结论:

黄金葡萄球菌在第二周对植入物培养的微生物负担明显高于其他药物.
这些发现可能解释了DAIR在PJI症状出现后3-4周内进行时的更高失败率.
这些结果支持这样一个观点,即DAIR可能对由黄金葡萄球菌引起的PJI效果较差.

关键词:

关节质整术是指关节质整术.生物膜是一种生物膜.微生物微生物周围假肢关节感染葡萄球菌奥雷乌斯病毒.

更多相关视频

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

相关概念视频

Microbial Morphologies

Microbial Morphologies

Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...

Evolution of New Traits in Microbes

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

The Skin Microbiota

The Skin Microbiota

The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...