基于数字电影的流量色度测试的应用,通过使用通用指示器的基于反的流量比计来检测酸定位的基于色彩的终点检测
Selass Kebede Olbemo1, Yosuke Sakai2, Masaki Takeuchi3,2,4
1Graduate School of Pharmaceutical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima, 770-8505, Japan. kselass@gmail.com.
概括
本研究引入了一种数字流色度测量方法,用于精确的酸定位. 该系统使用通用指示器和数字显微镜来检测pH值的变化,从而实现自动化和高通量终点的确定.
科学领域:
- 分析化学 分析化学
- 物理化学 物理化学
背景情况:
- 传统的酸定位通常需要手动检测终点,这可能是耗时和主观的.
- 使用通用指标提供了广泛的pH范围,但需要仔细观察才能准确地确定终点.
研究的目的:
- 开发和验证基于数字电影的流色测系统,用于在酸定位中自动检测终点.
- 通过使用通用指标建立一种高通量和多用途的pH测量分析方法.
主要方法:
- 建造了一个带有环静电和数字显微镜探测器的双通道流系统.
- 使用自定义的Visual Basic.NET程序进行了自动化分析,监测不同流量比率的通用指标的色调变化.
- 该系统通过对各种酸和的定位验证,包括对酸的逐步定位.
主要成果:
- 数字流色度测量方法根据快速的色调变化准确地确定了终点,与通用指示器的颜色过渡相关联.
- 该系统通过为等价点设置特定的色调标准,成功地对多价值酸进行了逐步定位.
- 对日本药典中氧和酸的测试结果符合规格,证明了该方法的准确性和可靠性.
结论:
- 开发的数字流色度测量是一种简单的,高吞吐量 (1滴定/分钟) 和多功能方法,用于酸滴定.
- 这种技术消除了基于等价点pH值的指示器更换的需要,提高了其适用性.
- 该自动化系统提供精确的pH测量和终点检测,适用于制药分析.
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