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在相对照显微镜中补偿半径效应,使用液晶显示器进行自适应电容器圆转移

Florian Nienhaus¹, Finn Burkhardt¹, Niels König¹

¹Fraunhofer Institute for Production Technology IPT, Aachen, Germany.

Microscopy research and technique

|January 17, 2025

概括

研究人员开发了一种适应性液晶显示器 (LCD) 圆形,用于微型晶体板 (MTP) 中相对比显微镜. 这种方法通过补偿半径效应,显著增强可观测区域,改善细胞培养成像.

科学领域:

生物医学成像技术生物医学成像技术
显微镜技术显微镜技术
细胞培养技术细胞培养技术

背景情况:

细胞培养器中的阴囊效应限制了相对比显微镜.
微型电压板 (MTP) 中的标准冷凝器环是静态的,不能弥补这种效应.
这种限制减少了细胞分析的可观察面积.

研究的目的:

开发一种使用相对比显微镜的方法来弥补MTP中阴囊效应.
通过调整冷凝器圆圈来增加MTP中的可观测面积.
为了提高细胞成像在microtiter板格式的可靠性和范围.

主要方法:

用透明液晶显示器 (LCD) 取代静态冷凝器圆环,能够进行自适应式圆环移动.
使用伯特兰透镜图像进行图像分析,以确定圆环和相环错位.
实现了线性回归模型,将图像分析数据转化为液晶显示器设置,以实现最佳对齐.
使用背景亮度算法可靠评估相位对比条件.

主要成果:

实现了相对比可观测面积增加8.3倍.
在24井MTP中增加了相位对比面积,在10倍放大时从5.0%增加到41.9%.
证明有效地补偿在MTP井中因半径引起的折射,不包括边缘.

结论:

适应性液晶圆环方法有效地弥补了MTPs中阴囊效应.
这种技术大大扩大了细胞培养中相对比显微镜的可用面积.
拟议的方法为需要相对照成像的高通量基于细胞的测定提供了显著的进步.

关键词:

液晶冷凝器环形是指液晶冷凝器的环形.图像分析图像分析菜单效应补偿菜单效应补偿微型板 (MTPs) 的使用阶段对比显微镜阶段对比显微镜

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