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相关概念视频

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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相关实验视频

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Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
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通过RNA干扰方法研究表皮细胞粘附.

Anamika Dutta1, Michele Calder1, Lina Dagnino2,3

  • 1Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.

Methods in molecular biology (Clifton, N.J.)
|January 17, 2025
PubMed
概括

本章详细介绍了一种在表皮细胞中使用RNA干扰进行基因沉默的方法,特别是通过小干扰RNA (siRNA) 转染. 它还提出了测试来研究基因淘汰如何影响细胞粘附性质.

关键词:
细胞粘附 细胞粘附皮肤表皮状癌细胞.基因沉默是一种基因沉默.氨酸细胞 (keratinocyte) 是一种细胞.这是一种RNA干扰.的 siRNA 转染.

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Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 基因沉默对于理解基因功能至关重要.
  • 表皮细胞在组织完整性和屏障功能中起着至关重要的作用.
  • RNA干扰 (RNAi) 提供了针对性基因淘汰的强大工具.

研究的目的:

  • 提供详细的协议,用于基因沉默在表皮细胞使用RNA干扰.
  • 描述传染小干扰RNA (siRNA) 寡核酸的方法.
  • 提出功能性试验,分析基因敲除对细胞粘附的影响.

主要方法:

  • 用于基因沉默的RNA干扰 (RNAi).
  • 通过转染介导的小干扰RNA (siRNA) 寡核酸的输送.
  • 功能测试用于评估基因淘汰后的细胞粘附性质.

主要成果:

  • 在表皮细胞中成功实施了siRNA介导的基因沉默协议.
  • 功能测试的演示,以评估细胞粘附的变化.
  • 从特定的基因淘汰中产生的生物后果的表征.

结论:

  • 描述的方法可以在表皮细胞中实现有效的基因沉默.
  • 提供的测试有助于研究基因功能,特别是与细胞粘附有关的基因功能.
  • 本章是研究人员在表皮生物学中研究基因功能的宝贵资源.