通过生物信息学分析探索与肝细胞癌预后相关的潜在关键基因和途径,随后进行实验验证
Xi Chen1, Jianhua Zhao1, Jiaming Shu1
1Department of Oncology, Jingdezhen First People's Hospital Jindezhen 333000, Jiangxi, China.
American journal of translational research
|January 17, 2025
概括
包括AURKA在内的四个关键基因对于肝肝细胞癌 (LIHC) 的进展至关重要. 这些基因表现出过度表达和低甲基化,影响患者的存活率,并为这种侵略性癌症提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 肝肝细胞癌 (LIHC) 是一种具有有限治疗选择的侵袭性癌症.
- 了解LIHC分子机制对于开发向疗法至关重要.
- 在LIHC中识别关键基因对于推进治疗策略至关重要.
研究的目的:
- 在LIHC.中识别差异表达基因 (DEGs).
- 确定参与LIHC病变的关键枢纽基因.
- 通过实验方法验证已识别的基因的作用.
主要方法:
- 对LIHC基因表达数据集的分析 (GSE84598,GSE19665).
- 使用R (limma包) 的微分表达式分析.
- 蛋白质-蛋白质相互作用网络建设 (STRING).
- 通过UALCAN,OncoDB,HPA,cBioPortal和卡普兰-梅尔分析进行验证.
- 实验验证包括AURKA在HepG2细胞中的敲除.
主要成果:
- 在LIHC中确定了180个DEG.
- 发现了四个关键的枢纽基因:AURKA,BUB1B,CCNA2和PTTG1.
- 在LIHC中观察到这些枢纽基因的显著过度表达和低甲基化.
- 在HepG2细胞中,AURKA knockdown抑制了细胞增殖,殖民地形成和伤口愈合.
- 枢纽基因的高表达与患者生存率差的相关性.
结论:
- 在LIHC病变发生过程中,AURKA,BUB1B,CCNA2和PTTG1具有关键作用.
- 这些基因代表LIHC的潜在生物标志物和治疗点.
- 这些发现为LIHC机制提供了新的见解,并指导了未来的研究.
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