Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

The novel antifungal agent NPD2560 perturbs the Rho1-centered signaling network to induce a cell wall integrity response.

Microbiology spectrum·2026
Same author

Orobas: A computational approach for scoring and analysis of quantitative chemical-genetic interactions from CRISPR-Cas9 screens.

STAR protocols·2026
Same author

Population-scale chemical response revealed by a barcoded yeast collection.

Nature communications·2026
Same author

Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.

Cell·2026
Same author

Expanding TheCellMap.org to visualize a genome-scale genetic interaction network for a human cell line.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jul 6, 2026

Identification of protein complexes with quantitative proteomics in S. cerevisiae
11:12

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Published on: March 4, 2009

13.2K

基于细胞图像的发芽酵母空间时空蛋白质组学协议.

Athanasios Litsios1, Myra Paz David Masinas1, Helena Friesen1

  • 1Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.

STAR protocols
|January 17, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种活细胞成像协议,用于跟踪整个芽酵母细胞分裂周期中的蛋白质变化. 这种方法可以进行时空蛋白质组分析,用于研究细胞周期过渡.

关键词:
细胞生物学 细胞生物学显微镜的使用方法一个单细胞的单细胞.系统生物学 系统生物学

更多相关视频

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.3K
Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays
05:57

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

763

相关实验视频

Last Updated: Jul 6, 2026

Identification of protein complexes with quantitative proteomics in S. cerevisiae
11:12

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Published on: March 4, 2009

13.2K
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.3K
Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays
05:57

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

763

科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 显微镜的使用方法

背景情况:

  • 细胞分裂周期在真核生物中涉及蛋白质定位和丰度的动态变化.
  • 这些蛋白质动态对于调节细胞分裂期间的关键转变至关重要.

研究的目的:

  • 介绍一个详细的协议,用于时空蛋白质组分析在芽酵母细胞分裂周期期间.
  • 为了使研究人员能够可视化和量化活细胞中的蛋白质行为.

主要方法:

  • 开发一个活细胞成像协议.
  • 菌株构建和培育芽酵母的方法.
  • 显微镜和随后的图像分析为蛋白质组数据.

主要成果:

  • 描述了一个用于时空蛋白质组分析的综合性协议.
  • 该协议允许在细胞周期期间研究蛋白质动态.
  • 突出了该协议对各种遗传和环境条件的适应性.

结论:

  • 提出的协议有助于在细胞分裂周期期间对蛋白质组进行深入分析.
  • 这种方法为了解发芽酵母细胞循环调节提供了有价值的工具.
  • 该协议的多功能性使其适用于各种研究场景.