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植物BCL-DOMAIN HOMOLOG蛋白在SWI/SNF复合体稳定性中起着保留作用
Joan Candela-Ferre1, Jaime Pérez-Alemany1, Borja Diego-Martin1
1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia 46022, Spain.
概括
植物BCL-DOMAIN HOMOLOG (BDH) 蛋白质是关键的SWI/SNF复合体子单元,对于皮细胞延长至关重要. 这些蛋白质稳定ARP异构体,确保SWI/SNF在真核生物中的活性.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- SWItch/糖非发酵 (SWI/SNF) 复合体是依赖ATP的染色质重塑剂,对真核细胞的核功能至关重要.
- 植物BCL-DOMAIN HOMOLOG (BDH) 蛋白质是植物SWI/SNF复合体的保存子单元,影响着色素的可访问性和发育.
研究的目的:
- 综合描述BDH突变体,阐明BDH在植物发育中的作用.
- 研究SWI/SNF复合体内BDH蛋白的分子功能和进化保存.
主要方法:
- 对Arabidopsis中bdh突变的分析,以研究 hypocotyl细胞延长.
- 对BDH蛋白质的域分析和在真核生物中进行的遗传学比较.
- 使用酵母Rtt102和Arabidopsis ARP7.7进行结构预测建模和功能测试.
主要成果:
- 突变bdh表现出缺陷的 hypocotyl 细胞延长,突出显示了 BDH 在这个过程中的作用.
- 在BDH中确定了一种特定于植物的N-终端域和一个保存的β-hairpin域,后者与哺乳动物BCL7有关.
- 酵母Rtt102是BDH/BCL7的结构同源,与Arabidopsis ARP7相互作用,并部分拯救了bdh表型.
- BDH稳定ARP4-ARP7异构体,影响SWI/SNF复合物的组成和活性.
结论:
- 通过其在SWI/SNF复合体中的作用,BDH蛋白对植物中低细胞细胞延长至关重要.
- β-毛域是SWI/SNF子单元的进化保存特征,对于ARP异构体稳定性和真核生物的复杂功能至关重要.
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