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Updated: May 5, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
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透性基准测试:用于比较,玻璃和活体测量的指南

Christian Jorgensen1,2, Raleigh M Linville3, Ian Galea4

  • 1School of Medicine, Pharmacy and Biomedical Sciences, Faculty of Science & Health, University of Portsmouth, Portsmouth PO1 2DT, Hampshire, U.K.

Journal of chemical information and modeling
|January 17, 2025
PubMed
概括

这项研究提供了指导方针,用于在计算,体外和体外方法中对细胞透性数据进行基准测试. 准确的透性测量对于药物输送和了解疾病至关重要.

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Methods for Studying Drug Absorption: In vitro01:16

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In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
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In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
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Equivalence: In Vitro and In Vivo Bioequivalence

Bioequivalence studies are crucial in evaluating whether new drugs can match an approved one regarding pharmacological effects and clinical performance. These studies test if drugs, despite different dosage forms, share identical plasma concentration-time profiles. Three types of equivalence are central to these studies: chemical, pharmaceutical, and therapeutic. Chemical equivalence indicates that two or more drug products contain identical active ingredients in equal amounts. Pharmaceutical...
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Drug Product Performance: In Vitro–In Vivo Correlation01:20

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In pharmaceutical development, it's crucial to establish a predictive in vitro–in vivo correlation (IVIVC) for two or more formulations to gain a comprehensive understanding of release properties. IVIVC reduces the need for costly in vivo studies and facilitates the establishment of meaningful dissolution specifications with significant cost savings and decreased regulatory burden. Furthermore, a meaningful IVIVC should predict Cmax and AUC within 20%, aligning with FDA guidance while...
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