蛋膜的亚临界水解及其物理化学特性
Kaipeng Cai1, Bolin Hou1, Yayun Hu1
1College of Food Science and Engineering, Northwest A&F University, Yangling, PR China.
Food chemistry
|January 17, 2025
概括
亚临界水 (SW) 处理有效地溶解卵膜 (ESM),与酸水解相比,产生更高的蛋白质和生物活性化合物. 这种绿色方法可以从ESM开发具有增强抗氧化性能的功能性食品.
科学领域:
- 生物材料科学 生物材料科学
- 绿色化学 绿色化学
- 食品科学 食品科学 食品科学
背景情况:
- 蛋膜 (ESM) 是一种具有有限应用的不溶性生物材料.
- 开发溶解ESM的方法对于其利用至关重要.
研究的目的:
- 使用绿色亚临界水 (SW) 工艺制备可溶性ESM.
- 为了比较SW水解与传统酸水解的功效.
- 为了研究SW温度对化物成分和生物活性的影响.
主要方法:
- 在不同温度 (180-210°C) 下,ESM的亚临界水 (SW) 水解.
- 为了比较,ESM的酸性水解.
- 分析蛋白质,,糖氨基甘氨酸 (氨酸,二硫酸) 和原蛋白含量.
- 扫描电子显微镜 (SEM) 和SDS-PAGE用于结构和分子分析.
- 评估自由基清除活动.
主要成果:
- 在180-210°C的SW水解产生了最佳的蛋白质 (40.57毫克/克), (20.36毫克/克),氨酸 (8.19毫克/克) 和氨酸硫酸盐 (11.68毫克/克).
- SW水解表明蛋白质水解和总自由氨基酸含量明显高于酸水解.
- 与酸性水解剂相比,SW水解剂的水解程度增加,自由基清除活性更高.
- SEM证实了ESM纤维的破坏,而SDS-PAGE显示了SW水解剂中的低分子量.
结论:
- 亚临界水 (SW) 是一种绿色和有效的溶解卵膜 (ESM) 的方法.
- 来自SW的ESM水解物具有更高的生物活性化合物产量和增强的抗氧化活性.
- 用SW处理的ESM水解剂显示出开发功能性食品和活性生物材料的巨大潜力.
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